electron spin resonance esr analysis equipment Search Results


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Magnettech GmbH electron paramagnetic resonance (epr) spectroscope miniscope ms5000
Electron Paramagnetic Resonance (Epr) Spectroscope Miniscope Ms5000, supplied by Magnettech GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genovis Inc electron spin operator
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Verlag GmbH high-frequency epr, ese, and endor spectroscopy
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Enzo Biochem 1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium chloride
Effects of siASK1 on superoxide production and TNFR endocytosis. A: the CAT1H signal in the media following TNFα exposure was not altered by siASK1, demonstrating that extracellular superoxide production in response to TNFα is not ASK1 dependent (n = 3). B: endocytosis of TNFR in VSMCs was impaired by siASK1. Bar graphs reflect fluorescent dots per cell. *P < 0.05 vs. siControl 0 min. †P < 0.05. C: representative images show that TNFR endocytosis (white dots) is reduced by siASK1. Results are representative of at least three independent experiments. D: TNFα-induced intracellular superoxide production is attenuated by siASK1. Total intracellular superoxide production was quantified using TMH. Representative electron spin resonance spectra for TMH (left) and means ± SE data (right). *P < 0.05 vs. siControl. †P < 0.05 (n = 4). ASK1, apoptosis signal-regulating kinase 1; CAT1H, <t>1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium</t> chloride; TMH, 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine; TNFR, tumor necrosis factor receptor; VSMC, vascular smooth muscle cell.
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SPI Supplies em grids spi supplies coated grids formvar®/carbon, 200 mesh, nickel
Effects of siASK1 on superoxide production and TNFR endocytosis. A: the CAT1H signal in the media following TNFα exposure was not altered by siASK1, demonstrating that extracellular superoxide production in response to TNFα is not ASK1 dependent (n = 3). B: endocytosis of TNFR in VSMCs was impaired by siASK1. Bar graphs reflect fluorescent dots per cell. *P < 0.05 vs. siControl 0 min. †P < 0.05. C: representative images show that TNFR endocytosis (white dots) is reduced by siASK1. Results are representative of at least three independent experiments. D: TNFα-induced intracellular superoxide production is attenuated by siASK1. Total intracellular superoxide production was quantified using TMH. Representative electron spin resonance spectra for TMH (left) and means ± SE data (right). *P < 0.05 vs. siControl. †P < 0.05 (n = 4). ASK1, apoptosis signal-regulating kinase 1; CAT1H, <t>1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium</t> chloride; TMH, 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine; TNFR, tumor necrosis factor receptor; VSMC, vascular smooth muscle cell.
Em Grids Spi Supplies Coated Grids Formvar®/Carbon, 200 Mesh, Nickel, supplied by SPI Supplies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Stanwix House individual electronic spin in diamond
Effects of siASK1 on superoxide production and TNFR endocytosis. A: the CAT1H signal in the media following TNFα exposure was not altered by siASK1, demonstrating that extracellular superoxide production in response to TNFα is not ASK1 dependent (n = 3). B: endocytosis of TNFR in VSMCs was impaired by siASK1. Bar graphs reflect fluorescent dots per cell. *P < 0.05 vs. siControl 0 min. †P < 0.05. C: representative images show that TNFR endocytosis (white dots) is reduced by siASK1. Results are representative of at least three independent experiments. D: TNFα-induced intracellular superoxide production is attenuated by siASK1. Total intracellular superoxide production was quantified using TMH. Representative electron spin resonance spectra for TMH (left) and means ± SE data (right). *P < 0.05 vs. siControl. †P < 0.05 (n = 4). ASK1, apoptosis signal-regulating kinase 1; CAT1H, <t>1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium</t> chloride; TMH, 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine; TNFR, tumor necrosis factor receptor; VSMC, vascular smooth muscle cell.
Individual Electronic Spin In Diamond, supplied by Stanwix House, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SPI Supplies conductive silver paint
Effects of siASK1 on superoxide production and TNFR endocytosis. A: the CAT1H signal in the media following TNFα exposure was not altered by siASK1, demonstrating that extracellular superoxide production in response to TNFα is not ASK1 dependent (n = 3). B: endocytosis of TNFR in VSMCs was impaired by siASK1. Bar graphs reflect fluorescent dots per cell. *P < 0.05 vs. siControl 0 min. †P < 0.05. C: representative images show that TNFR endocytosis (white dots) is reduced by siASK1. Results are representative of at least three independent experiments. D: TNFα-induced intracellular superoxide production is attenuated by siASK1. Total intracellular superoxide production was quantified using TMH. Representative electron spin resonance spectra for TMH (left) and means ± SE data (right). *P < 0.05 vs. siControl. †P < 0.05 (n = 4). ASK1, apoptosis signal-regulating kinase 1; CAT1H, <t>1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium</t> chloride; TMH, 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine; TNFR, tumor necrosis factor receptor; VSMC, vascular smooth muscle cell.
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Effects of siASK1 on superoxide production and TNFR endocytosis. A: the CAT1H signal in the media following TNFα exposure was not altered by siASK1, demonstrating that extracellular superoxide production in response to TNFα is not ASK1 dependent (n = 3). B: endocytosis of TNFR in VSMCs was impaired by siASK1. Bar graphs reflect fluorescent dots per cell. *P < 0.05 vs. siControl 0 min. †P < 0.05. C: representative images show that TNFR endocytosis (white dots) is reduced by siASK1. Results are representative of at least three independent experiments. D: TNFα-induced intracellular superoxide production is attenuated by siASK1. Total intracellular superoxide production was quantified using TMH. Representative electron spin resonance spectra for TMH (left) and means ± SE data (right). *P < 0.05 vs. siControl. †P < 0.05 (n = 4). ASK1, apoptosis signal-regulating kinase 1; CAT1H, 1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium chloride; TMH, 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine; TNFR, tumor necrosis factor receptor; VSMC, vascular smooth muscle cell.

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNFα receptor endocytosis

doi: 10.1152/ajpheart.00741.2018

Figure Lengend Snippet: Effects of siASK1 on superoxide production and TNFR endocytosis. A: the CAT1H signal in the media following TNFα exposure was not altered by siASK1, demonstrating that extracellular superoxide production in response to TNFα is not ASK1 dependent (n = 3). B: endocytosis of TNFR in VSMCs was impaired by siASK1. Bar graphs reflect fluorescent dots per cell. *P < 0.05 vs. siControl 0 min. †P < 0.05. C: representative images show that TNFR endocytosis (white dots) is reduced by siASK1. Results are representative of at least three independent experiments. D: TNFα-induced intracellular superoxide production is attenuated by siASK1. Total intracellular superoxide production was quantified using TMH. Representative electron spin resonance spectra for TMH (left) and means ± SE data (right). *P < 0.05 vs. siControl. †P < 0.05 (n = 4). ASK1, apoptosis signal-regulating kinase 1; CAT1H, 1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium chloride; TMH, 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine; TNFR, tumor necrosis factor receptor; VSMC, vascular smooth muscle cell.

Article Snippet: For quantification of superoxide production, the membrane-impermeable electron spin resonance (ESR) probe 1-hydroxy-2,2,6,6-tetramethylpiperidin-4- yl -trimethylammonium chloride (CAT1H; Enzo Life Sciences, Farmingdale, NY) and cell-permeable spin probe 1-hydroxy-4-methoxy-2,2,6,6-tetramethylpiperidine (TMH; Enzo Life Sciences) were used.

Techniques: Electron Paramagnetic Resonance